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Human HGF Protein

Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

Catalog No. Product Name Size List Price (US$) Quantity
BP004703 Human HGF Protein with the C-His Tag 100UG 345.00
BP004703 Human HGF Protein with the C-His Tag 500UG 1150.00
BP004702 Human HGF Protein with the C-hFc Tag 100UG 345.00
BP004702 Human HGF Protein with the C-hFc Tag 500UG 1150.00
Description

BP004703: Human HGF Protein with the C-His Tag

Source: The recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The recombinant Human HGF protein with the His tag at the C-terminus has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human HGF protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HGF R protein with the C-hFc tag with the EC50 of 9.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004702: Human HGF Protein with the C-hFc Tag

Source: The recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The recombinant Human HGF protein with the hFc tag at the C-terminus has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human HGF R protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HGF protein with the C-hFc tag with the EC50 of 26.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human HGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following HGF Protein :
HGF Protein
Human HGF Protein
Biotinylated Human HGF R Protein
Cynomolgus HGF R Protein
Human HGF R Protein
Mouse HGF R Protein
Cynomolgus HGFA Protein
HGF A Protein
Human HGFA Protein
Mouse HGFA Protein
HGF B Protein

Syd Labs provides the following HGF Antibody :
HGF Antibody

Syd Labs provides the following HGF ELISA Kit :
Human HGF ELISA Kit

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